Other mistake: Near the beginning of the movie, Milla Jovovich reads a note that has been left on the desk. She didn't write it, as they demonstrate in the film. However, when they do show the person who wrote the note composing it, the handwriting is different from that in the note from the earlier scene. (00:10:20 - 01:12:25)
Continuity mistake: Near the end of the film, on the train ride out of the Hive, Rain is quite close to becoming undead. When she speaks, we notice her teeth are still "movie-star white". However, a few shots later, when she becomes a zombie, she sort of hisses where you can see it has taken what couldn't have been more than a few mere minutes for her teeth to become brown and discolored.Sammi
Revealing mistake: When the group is being attacked in dining hall B, they are trying to get the doors open. J.D. is firing at two zombies and turns away to open the doors himself because Kaplan keeps forgetting the code. If you look closely, one of the zombies reacts a second late from being shot down. (00:44:40)
Revealing mistake: On the DVD commentary, the director says that he only had a handful of the experiment cubes in Dining Hall B and used cardboard cutouts for all the others. This is most obvious when Rain is walking through the hall alone to check on the first zombie: the cubes in the distance don't match the rigid rows of the ones in the foreground. (00:38:20)Phoenix
Continuity mistake: Near the end, when Alice, Rain and Matt are talking to the "Red Queen" in the virus lab, there is a wall-mounted LG Flatron TFT display where the Red Queen shows surveillance footage, etc. One can see an inventory type barcode label in the bottom centre of the screen. The camera cuts away briefly, and when the camera shows the display again, the barcode label is gone and the LG logo is in its place. (01:14:50)Shaun Ewing
Continuity mistake: When Alice escapes the first zombie dog and locks herself in the room, a zombie approaches her and she kicks him down. When the zombie falls you can see his tie is partially outside of his suit. When it cuts to Alice reaching for his gun, the tie is tucked inside his suit. (00:47:20)Mortug
Continuity mistake: The way the T-Virus capsule hits the ground is the same each time they show it, however when it's on the ground and motionless, the position is changes. During the flash back at the end that Spencer is having, the capsule is all over the place. However at the beginning, the capsule is just in a little straight pile on the floor.