Revealing mistake: When the Red Queen scans the doctors in the lab, it displays "Dr. Anna Bolt." This is the name of the actress, not the character. (00:05:10)
Other mistake: In the beginning when Spence breaks the T-Virus vial, its fumes can be seen entering the ventilation system from holes on the wall. Shortly after though, when Dr. Blue, Dr. Brown and Dr. Green are trapped in the flooded room, the former of them claims it is "sealed." (00:03:05)
Audio problem: Near the middle. When Matt is searching through the office room where his sister Lisa worked, you can hear his shoes clicking as if he is on a hard surface floor, but the floor is covered in carpet throughout the office. (00:49:00)
Continuity mistake: When Alice and Matt run to meet up with Spencer, Kaplen, and Rain, Alice screams "Don't shoot!" and Spencer, Matt, and Alice try to close the door from the zombies. A zombie then grabs the inner part of Spencer's arm, then a shot moves showing the zombie holding onto Spencer's arm from the bottom, then back to the inner part. (00:55:45)
Factual error: When Alice has the pistol she got from the zombie she took out, she uses it to do away with the zombie dogs. As she fires the last of the rounds, the camera looks down to follow the fall of the casing. As it falls, it's on a slight tilt, about 5°. Physics says that it will bounce and tumble on the floor after it hits, then come to rest. Instead, when it hits, it immediately goes to a rest position with little movement. (00:48:20)
Audio problem: In one of the scenes right before the solider get killed by the lasers and the alarm is sounding, the commander says to everyone "Hold your positions, everyone stay calm" but his lips aren't moving. (00:31:20)
Suggested correction: Actually if you look closely they are. You can see the white of his teeth when he talks.
Factual error: The case containing the virus requires a four digit code to open it. As demonstrated by the operative that opened it to give himself the antidote near the train. Yet Alice closes the case, and later opens it without entering a code.
Continuity mistake: At the start of the film, the scientists are attempting to breach the window with an axe. Later in the same lab in the movie, Alice picks up an axe, even though it was shown before being wielded by the zombie.
Factual error: When Red Queen is explaining about the T-Virus they say that fingernails and hair continue to grow after death. This is not correct, While it appears that they grow, its actually down to the tissue drying out and retracting. An article on the topic can be found here: https://www.bbc.com/future/article/20130526-do-your-nails-grow-after-death A super computer that is knowle. (00:56:40)
Suggested correction: The T-virus causes the hair and nails to keep growing. The nails cause scratches that can increase infection, so it benefits the T-virus.
That is not what was said at all. In the film it states that even in death, the body remains active and that hair and fingernails continue to grow, news cells are produced, and the brain holds a small electrical charge that takes months to dissipate (all of which are false). Then the T-virus provides a massive jolt to growing cells and the brain to reanimate the dead.
She says those things after they ask her what those things are. She then starts to explain how the T-virus works. She doesn't say a dead body always keeps active, she says a dead body infected with the T-virus is still active, regenerating cells, hair and fingernails continue to grow. In short, it reanimates the dead (to a degree). That's how I read it anyway.
Where is it stated that the T Virus actually does this? Its stated that the virus reanimates the body but not sure on the other.

Continuity mistake: When the first laser passes through it hits the one guy in the wrist, however we see him on the floor missing half of his fingers and hand instead. (00:31:30)

Continuity mistake: In the laser corridor, the team leader is stood upright when the lights comes on, but as the camera changes to a front view, he is crouched. (00:30:10)
Suggested correction: It's easy to miss, but there is a quick insert-shot in-between these two shots that shows the team leader crouching down suddenly. (The camera is focused on the back of his head as he ducks down suddenly.) It can be seen in this video (https://www.youtube.com/watch?v=rOLe15-5Xhw&t=65s) around the 1-minute mark.
Continuity mistake: In the scene where Matt pulls Alice into the mansion at the beginning of the movie and the SWAT team comes through the windows, the main SWAT member pulls Alice aside and asks for her report. If you watch the scene Alice has one arm at her side and one across her chest. A split second later it shows her reflected in the eye lenses on the SWAT member's mask and Alice has her arms crossed in front of her. Another split second later they show Alice again and her arms are in the original position. (00:13:35)