Trivia: In the final scene when Milla Jovovich emerges from Racoon City Hospital into the ruined city, a newspaper blows past in the wind with the headline "The Dead Walk" - a blatant reference to George Romero's classic zombie flick "Day of the Dead" where a newspaper with the same headline blows past in the opening sequence.
Trivia: A subtle reference to the games: The name on the front of the transit train is 'Alexia'. This could be a reference to Alexia Ashford, an Umbrella researcher who appeared in 'Resident Evil: Code Veronica'. I'm also fairly certain the names 'Red Queen' and 'Hive' are attributed to Alexia's fanatical obsession with ants and the fact that the T-Virus, in the games, was supposedly discovered in an ant corpse.
Trivia: When Spence enters the secured room where the T-Virus was stored he suddenly experiences the flashback of him stealing it. This is remarkably similar to what happens to Ark Thompson in the game 'Resident Evil: Survivor'. He wakes up with no memory of who he is, fights his way into a lab complex and suddenly remembers everything before encountering a Virus experiment gone wrong. I don't think it was done intentionally but it's an interesting game reference nonetheless.
Trivia: All the cast and crew (including the producer) had to undergo a grueling one week of military training (which they dubbed their 'week of hell') under the supervision of a battle hardened ex-navy SEAL. By the end most people were naturally eager to give their trainer a little of his own medicine. Miss Jovovich (Alice) did: he's the zombie she head kicks into the test-tube cabinet and then steals the gun from (before fighting the zombie dogs). What sweet justice.
Trivia: Fans of the games were disappointed that no game characters appeared, however they are referenced twice at the end of the movie. The newspaper seen on the stand in the foreground as Alice leaves the hospital has a sub-headline mentioning the Special Tactics And Rescue Squad police unit, and a police car next to the one Alice gets the shotgun from has the S.T.A.R.S. emblem on the bonnet.