Revealing mistake: During the scene when the people at the pool think Chris is a car sitting on the diving board, just before Lois moves out of the shot before the scene changes, her lips disappear for a split second. It's really fast but still easy to notice even without pausing or slow motion.
Revealing mistake: After the fight between Stewie and Olivia at the concert hall (followed by their breakup), Simon tells Stewie that the act without Olivia is "over" as he turns to walk off the screen. However, before Simon is even partially out of the shot, he completely disappears. (00:18:45)
Revealing mistake: When Brian says "Well face it Peter, your attitude towards women isn't exactly enlightened", Brian's dog tag is the same color as his fur. It also stays still, even while Brian is moving his head. (00:11:10)