Other mistake: During the Poke-rap when the Poliwrath name is said, the Pokemon shown is actually Poliwag. Also, when the Graveler name is said, the Pokemon shown is actually Geodude.
Other mistake: Larvitar's weight is 158.7lb, but Ash can carry it in his backpack without any problems.
The March of the Exeggutor Squad - S1-E41
Other mistake: The Pokemon Exeggcute is shown evolving in to an Exeggutor naturally. But the Pokemon Exeggcute can only evolve into an Exeggutor with the use of a leaf stone. In the Japanese version, there is a mention that something in these woods causes Exeggcute to evolve without using the Leaf Stone (when Exeggcute evolves, James even mentions that there is no stone). However, as this was skipped in the English version, it creates a plot hole.
Other mistake: Assunta's Ivysaur attacks Sparky too early, before the battle even started, not to mention Assunta haven't given it any commands during the battle.
Bulbasaur and the Hidden Village - S1-E10
Other mistake: In the scene when Team Rocket tries to steal the Pokemon, Bulbasaur saves Oddish. Ash comes to help push Bulbasaur, and for a few frames you can see 2 sets of hand prints cut out where Ash's hands go.
What You Seed Is What You Get - S7-E1
Other mistake: When Poliwrath first appears in the Pokémon Trainer's Choice, its swirl mistakenly faces clockwise instead of counterclockwise. (00:12:13)

Mystery at the Lighthouse - S1-E13
Other mistake: When Professor Oak goes to rotate his monitor, his right index finger is intermittently not coloured in. (00:09:15)
Abra and the Psychic Showdown - S1-E22
Other mistake: When Ash says, "Without a Marsh Badge, I can't get into the Pokémon League!" the strips on Pikachu are missing. Also, Ash does not have gloves, but in the next shot, he does. (00:07:11)
Pokemon, I Choose You! - S1-E1
Other mistake: When Pidgey turns its head to see Ash with a Poké Ball, its head feathers beside its eyes are mistakenly coloured cream instead of brown. (00:11:36)
Mystery at the Lighthouse - S1-E13
Other mistake: When Ash and friends are first seen walking on a path, Pikachu's cheek pouches are missing. (00:01:14)
Other mistake: When the camera pans across the Pikachu when they are surprised, one of them is missing its cheek pouches while another is missing its left one. (00:03:06)

Mystery at the Lighthouse - S1-E13
Other mistake: When Brock turns his head to the costume, his nose is missing. (00:12:47)
Island of the Giant Pokemon - S1-E17
Other mistake: In the recap, when Ash, Misty, and Brock are worried about the cyclone approaching, the edges of Meowth's ears are mistakenly colored tan instead of black. (00:01:16)
Other mistake: When Ash, Misty, and Brock are worried about the cyclone approaching, the edges of Meowth's ears are mistakenly colored tan instead of black. (00:20:08)
Other mistake: When Ash and friends are first seen when a Ghost Pokémon laughs, the base on Pikachu's tail is missing. (00:07:47)
Other mistake: When Brock says, "Yeah. The season of love is when Butterfree lay their eggs," two Butterfree occasionally are missing feet, and another one occasionally has a part of its wings mistakenly colored black instead of white. (00:01:49)
Clefairy and the Moon Stone - S1-E6
Other mistake: When the camera begins panning back to show Seymour, Ash, Butterfree, Zubat, and Brock, Pikachu's mouth is missing. (00:11:18)

Fushigidane and Fushigidane! Regaining the Monster Balls!! - S7-E34
Other mistake: The English title card misspells Poké Balls as "Pokéballs." (00:02:41)
Protecting the Egg! The Life Born in a Storm! - S5-E49
Other mistake: When showing Team Rocket from the front after Ash is first seen using his shirt to help hatch the Egg, Meowth's charm has six lines instead of four. (00:01:53)
Other mistake: When Meowth shifts his eyes from his book about the trophy to the trophy, he has five lines on his charm instead of four. (00:04:11)
Answer: Yes it ran away because Layla hurt its feelings.