Continuity mistake: When Frost has the head pure-blood vampire on the beach, after he pulls out his first vampire tooth you see blood all over his mouth, but when it shows the close up of him pulling out the other tooth, there is no blood. (01:04:22)
Continuity mistake: When Blade gets back from the park after meeting with Frost and getting serum, he discovers Frost's men have been there, and sees Whistler. When Blade moves the tape, which is in a metal rotating stand, the stand rotates on its swivel, but in another shot it has only moved a little bit and hasn't rotated either. The tape would've been facing the other direction because the stand swivelled, but instead it remains in the same position. (01:12:30)
Continuity mistake: When Blade drops Karen off, she walks to her apartment and as she approaches the elevator a blonde woman walks up to its doors and stops, but in the following shot the blonde woman is now just coming into view and then stands behind the black woman instead of at the elevator doors. (00:32:15)
Continuity mistake: Frost says near the end of the film that the spirits of the twelve will awaken the Blood God. Fair enough, but when the prophecy is first translated, you hear twenty beeps as pictures of people pop up around the circle, implying that there are twenty people standing around it.
Continuity mistake: In the beginning of the movie when Frost leaves the meeting, as he leaves the sound from his shoes is delayed.