Continuity mistake: When Frost has the head pure-blood vampire on the beach, after he pulls out his first vampire tooth you see blood all over his mouth, but when it shows the close up of him pulling out the other tooth, there is no blood. (01:04:22)
Visible crew/equipment: Near the start when the vampire that's been burnt gets taken into hospital, watch the big glass cabinet as he gets wheeled in by 2 doctors - the crew and the boom mic are reflected in the glass. (00:10:59)
Continuity mistake: When Blade gets back from the park after meeting with Frost and getting serum, he discovers Frost's men have been there, and sees Whistler. When Blade moves the tape, which is in a metal rotating stand, the stand rotates on its swivel, but in another shot it has only moved a little bit and hasn't rotated either. The tape would've been facing the other direction because the stand swivelled, but instead it remains in the same position. (01:12:30)
Revealing mistake: When Blade is being put into the chamber so they can siphon his blood, there are straps for his neck and his wrists. He is placed into the chamber and his hands are placed in the wrist restraints, no one reaches for the neck restraint. In the next scene, the strap is around his neck.
Continuity mistake: When Blade drops Karen off, she walks to her apartment and as she approaches the elevator a blonde woman walks up to its doors and stops, but in the following shot the blonde woman is now just coming into view and then stands behind the black woman instead of at the elevator doors. (00:32:15)