Continuity mistake: In the subway, Blade and Quinn are fighting on the edge of a subway station for at LEAST two straight minutes as a train barrels by. In NYC, it takes a subway car one to two seconds to pass a stationary point when moving at normal speed. Even being generous and saying two seconds per car, what subway train has sixty cars in it? (normal is eight, I believe). (00:55:00)
Continuity mistake: When Blade is kicking butt in the club, he jumps into a shower, stabs the last guy then jumps onto a side ledge and has the sword in his right hand. When the camera cuts to Quinn then back to Blade, he is switching his sword from his left hand to his right. (00:08:45)
Continuity mistake: Right before the sword embeds the marble, it's on the right side of EDTA. When the sword handle opens it's above it. (01:51:30)
Continuity mistake: When Blade chops off Quinn's hand in the hospital, we see it fall down inches from Karen's face, as she is lying on her front on the floor. After the fight is over, Blade finds Karen lying on her back. It is unlikely that, being as incapacitated as she appears, she would have the strength or inclination to turn her whole body over. (00:14:04)
Continuity mistake: During the final battle, right after Frost's arm regenerates and he says "It's too late, Blade!", Blade begins swiping his sword at Frost rapidly. You can hear the sound of Blade's sword slicing into Frost's shirt, and even see numerous pieces of fabric from the shirt flying about in the air as Blade shreds off pieces. Yet, for the rest of the scene, Frost's shirt is almost completely undamaged, save for one or two small tears. This makes no sense, as we had just seen and heard Blade slicing off numerous pieces of fabric from the shirt. The pieces seen flying through the air were actually added later using computer effects to make the scene look more dramatic, thus creating the continuity problem.
Continuity mistake: When Karen turns on the UV lamp the first time and starts to burn Pearl (the enormous record-keeper), watch closely. In the first shot from the front, Pearl has no burns yet and the air is clear. It immediately cuts to an over-the-shoulder shot and not only does Pearl's arm instantly have burns on it (that had no time to appear between cuts), but there's also smoke in the air that wasn't there in the previous shot. (And also had no time to appear between cuts).
Continuity mistake: When Whistler is giving Blade the injection, watch the vial. It starts full, then in a close up, you can see the serum level drop. Then the camera goes to a wide shot, and the fluid level is almost full and dropping, The next close up, and the serum level falls to completely empty.
Continuity mistake: There is a scene where Blade and Frost are having a discussion sitting across from one another. The shot changes back and forth from Blade to Frost as they speak. On the table you can see a pack of cigarettes next to frost that changes position in every shot and even disappears entirely in one of the shots.
Continuity mistake: When Blade throws the darts full of EDTA into Frost at the end of the movie, they all just sit there in Frost's flesh, not pumping the blue fluid into him (this is especially evident after Blade kicks the final one into Frost's face). This is a blooper in and of itself, because as we saw earlier (as Blade fought two vampire guards and killed them with the same darts), the darts quickly pump the fluid out upon impact. What's more, all the darts are suddenly completely empty in the zoom-out shot of Frost's body bubbling and expanding from the reaction to the EDTA.
Continuity mistake: When Blade is escaping from the police, after he jumps across the building he uses a red door as a sheild. The police shoot the red door and it splinters. After the doctor pops his shoulder back in, the cops shoot the door again but there are no visible marks from the bullets before and instead of a wooden door it's now metal.