Continuity mistake: Bart's guitar is lefthanded. So when the righthanded Otto plays it on the bus, the guitar is flipped over, with the little speaker on the top. But when Otto is playing it later in the garage, it is a normal righthanded guitar, with the little speaker on the bottom.
Continuity mistake: When Skinner is calmly driving the bus on the way to school, they have obviously gone past a number of power lines (you can see that they have because you can see them in the background). However, when Skinner is trying to get onto the main road, the power lines have disappered from the background. (00:12:45)
Continuity mistake: At the very end of the episode, when the kids are lined up to wait to see whether Otto is back driving the bus, there are several shots that switch back and forth between a direct view of the kids and a shot from behind. Lisa changes position between shots. (00:21:15)
Continuity mistake: When Otto is playing the guitar with bart in the middle of the night, there are close-ups of Otto whenever he is singing. You can see that the wall behind him is plain. However, when there is a wide shot of otto and bart, the wall behind otto has two tables leaned against them. (00:15:50)