Other mistake: In the scene where Dr. Isaacs is in the teleconference with the Umbrella Board of Directors, there are several glasses of water sitting in front of each individual hologram. At the end of the teleconference, the holograms of the board members disappear, but none of the glasses follow suit. This tells us that the glasses of water were physically in the room with Dr. Isaacs, for absolutely no reason, given all the other attendees weren't really there.
Other mistake: In the end fight scene between Dr. Isaacs and Alice, she bumps into the water cocoon and awakes one of her clones only to have it die a few seconds later. When she goes to cover up the body, the clone can be seen wearing a wedding ring - they don't incubate the bodies wearing jewelry in an embryonic state.
Other mistake: In order to use the vials that have either the T-virus or the anti-virus it has to be loaded into a special gun-like tool, similar to a caulking gun. A plunger is then set, and released with a trigger, again, like a caulking gun. The problem is that when the vials are seen, the caps on either end are solid, with no hint of an opening for the fluid to get out and into the body that it is being administered to.
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