Factual error: When the train passes the road crossing where it should have stopped - at 6pm - it is very bright daylight, and the sun is high in the sky (which can be seen from the shadows, among others those cast by the car on the road). The sun is never so high at 6pm in western Turkey/eastern Greece. That scene has been shot earlier in the day.
Continuity mistake: In the close-up where we see Krinelcu get shot, he is resting his legs on a flat surface just below our view. That is clear on the position of his legs and the movements of his body. He is kneeling on a floor. But in the clips we have seen of the house it is clear that the poster is painted on a completely flat building. There is no ledge for him to be resting on. Also in the shot where he falls from the lighted opening, no ledge is visible.
Continuity mistake: During the helicopter sequence towards the end of the film, we see a view from the pilot's seat in the helicopter and Bond can be seen running directly below it. In the next shot, however, Bond is nowhere near the helicopter.
Continuity mistake: During the boat chase scene, Morzeny is first seen riding in a boat with a small wooden mast arising from the middle of the windshield (similar to Bond's boat). The mast is present in most shots, but at one point, when he tells the boat "SPECTRE 3" to move over so his boat can come through, the mast is gone, replaced by a small antenna.
Continuity mistake: In the scenes in which Tatiana is in the back of the flower truck and aboard the boat she is wearing a light blue top and gold skirt. Yet in the shot in which she moistens her finger and holds it up to the wind she is also wearing a vest that matches the skirt. But, the only luggage they brought on board from the truck are Bond's attache case and the Lektor.
Continuity mistake: Q demonstrates to Bond how to properly rotate the latches on his briefcase to arm or disarm the exploding tin of talcum held inside. Q then asks Bond to open and close the latches, to be sure he knows how to do it. Bond closes the first latch without any trouble, but the second latch will not engage, and Sean Connery does his best to 'keep the scene alive' by ignoring the recalcitrant latch and tries to obscure the faulty mechanisim by casually placing his hand over the latch. As Bond turns to leave M's office with the briefcase, the latch is still open, yet as Bond reaches the doorway the scene cuts to the opposite view of the door and the latch is now securely closed. (00:22:05)
Continuity mistake: When Bond tells Sylvia that he has to make a phone call, Sylvia passes Bond his shirt and he walks over to the car. Bond then picks up the phone in the car and starts to put his shirt on. After a quick cut to Miss Moneypenny, we then return to Bond and he has now managed to put his shirt completely on and buttoned, one-handed, in the space of just five seconds. (00:18:30)
Other mistake: When Bond returns to his room and places the rifle kit back in his briefcase, he fails to deactivate the booby trap before opening the case, but nothing happens. (00:49:40)
Continuity mistake: On the train Bond gives Tatiana a powder blue negligée, the upper portion is lace. She puts it on and they snuggle a bit, then Bond is called out by Kerim about something. Bond returns a few minutes later and she has on a totally different negligée that has no lace on it but is still powder blue. (01:06:40)
Factual error: When Bond arrives in Istanbul, the opening shot for the scene shows him arriving on the American-based Pan Am flight. Pan Am, or any American-based airliner, would most likely not have a flight going from London, UK to Istanbul, Turkey. Bond would have been most likely taking either a British-based airliner or a Turkish-based airliner. This error is more or less heavy handed product placement that wouldn't really happen in the real world, unless Bond flew from London to New York to Istanbul and that certainly would never be approved by a British government agency for one of its employees. (00:24:35)
Character mistake: In the scene at Kerim Bey's office, when the bomb goes off, we see a heavy desk topple over. Bey says he was saved because he was relaxing on the couch with a girl. But he wasn't at the desk before either. He was sitting in some sort of sofa arrangement in a different corner of the room. We don't even see the desk before the bomb goes off. (00:32:05)