Audio problem: When Bond is involved in a high-speed chase on the way to Miss Taro's house, his wheels are squealing like crazy, but he's on a dirt road. (00:48:10)
Continuity mistake: After Bond gets picked up from the airport, evades a car, then fights with the driver, at one point he pulls back his right arm, but then the angle changes, and he hits him with his left one. (00:20:20)
Revealing mistake: When James Bond has a spider on his arm, one can see he's under a glass plate, and the spider on top of the plate. (00:41:55)

Continuity mistake: When Bond kills a patrolman on the island, his shirt is dry around his shoulders right after rising out of the water. (01:08:40)
Continuity mistake: The hearse that was involved in Strangways' death and later chases Bond is a 1937 or 1938 Lasalle, with the pod style headlights mounted on stalks on either side of the radiator. The car that drives off the cliff and explodes is a later model car, with the headlights mounted flush into the fenders.It is very easy to spot while the burning car is rolling down the side of the cliff. (00:48:50)
Audio problem: When Bond and Honey are on the beach the hoods of Dr No come by boat. On the boat there is a guy shouting through a megaphone. As they sail away again the man waves the megaphone to his comrades and says "full speed ahead". Yet his voice sounds identical to when he was speaking through the megaphone, though he isn't holding it to his mouth anymore. (01:04:00)
Continuity mistake: During the evening meeting in the bar we can see the band and a singer, but movement of his lips (despite that they were trying to hide them behind a big microphone) don't match what is sung. (00:30:15)
Continuity mistake: When Bond gets directions to Miss Taro's house, her instructions to Bond conclude with "Follow the road up the hill, down the other side, and two miles further on, on the left Magenta Drive, 2391," but when Bond is in her house later on, while calling for a taxi, the house number changes and he gives it as "2171 Magenta Drive". Miss Taro even nods to confirm it. (00:47:30 - 00:51:20)

Continuity mistake: Bond sits down by a table, puts some cards down, and pulls his gun out. He's wearing a tie, but when we see a closeup of him screwing on the silencer, his tie disappears. (00:54:40)
Continuity mistake: During the car chase on the way to Miss Taro's house, we can see the same shot of the cars at the turn three times. (00:48:15)
Continuity mistake: A car chase early on has a cutaway to the speedometer of the villain pursuing Bond, of a dashboard totally and obviously different to the actual one on the car. The director apparently knew it, but they were low budget and had no choice. (00:18:25)
Revealing mistake: When Dr. No crushes the Buddha in his powerful deformed hand, watch when he first touches it. It is made out of rubber. (01:29:00)
Audio problem: When the powerboat shoots at the island, afterward, when the guy with the speakerphone tells them that he would be back later on, he then turns to the driver and tells him to move on, but when he says that, it sounds as though he's still talking in the microphone even thought the microphone is at his side. (01:04:20)

Continuity mistake: When Bond enters the office of Universal Imports he throws his hat on the hat rack. After his meeting with M, he retrieves his hat from the hat rack, but it has moved to a different hook. (00:09:25 - 00:14:05)
Continuity mistake: When Quarrel gets cut by the broken flash bulb, he has a lot of blood on his face and the rag. The next scene he's fine with no blood. (00:32:45)
Revealing mistake: James Bond and his two friends are hiding underwater while Dr. No's thugs search the river with dogs. Bond had very quickly cut three hollow bamboo stalks to use to breathe underwater through the stalks. However, you can see the sections in the bamboo, so the closed stalk sections, separated by the natural divider, would not allow air to pass through. Bond hadn't cleared through the sections with a thin pole, so these would not have been usable as breathing tubes without more work. (01:10:00 - 01:12:00)
Plot hole: When Bond is escaping through the metal ducts, presumably of Dr No's air-conditioning, he is deluged with torrents of water. He then leaves the pipe work through a metal grille into a passageway - why didn't the water leave the same way? (01:33:05)
Plot hole: When Bond, Quarrel and Felix sail to Crab Key, Felix says that it's 48 hours to the moonshot. Bond replies that they'll "Be back in 12, if not then it's your beat and you'd better bring your marines with you". We know that Bond was still on the island when the rocket took off (it's when he kills Dr. No) and yet it's only when He and Honey are sailing AWAY from the island that Felix shows up with the marines. Why did he wait the full 48 hours (longer even as the moonshot had already occurred) when it was obvious something was wrong when Bond hadn't come back after the 12 or so hours as agreed? (00:57:45 - 01:43:40)
Factual error: Bond is worried that Dr. No has picked up the sails of Honey's boat on his radar, as indeed the good doctor has (in the movie), but radar systems cannot detect cloth or a wooden mast. (01:01:40)
Continuity mistake: When Bond goes to Miss Taro's house, as he enters, one of the blinds is turned on the door, but they were all straight before. (00:49:50)
Answer: In Goldfinger, Q says "It has not been perfected, out of years of patient research, ENTIRELY for that purpose, 007. And incidentally, we'd appreciate its return, along with all your other equipment, INTACT for once, when you return from the field."
Myridon