Continuity mistake: When we see the extreme close up of the robotic arm picking up the blue vial, the grip is uniform and parallel to the edge of the sliver end cap on the vial. When we see the vial a second later, the arm's grip has changed and is no longer parallel or uniform on the vial. (00:02:10)
Continuity mistake: In the beginning when the the cylinders are being placed in the holders, in one close-up you can see the middle cylinder being placed and that there is no cylinder to the right of it. When it cuts a cylinder has suddenly appeared. (00:02:20)
Continuity mistake: When the person is bumped into and spills coffee on himself, the coffee stains on his shirt changes between the shots. (00:03:05)
Continuity mistake: The way the T-Virus capsule hits the ground is the same each time they show it, however when it's on the ground and motionless, the position is changes. During the flash back at the end that Spencer is having, the capsule is all over the place. However at the beginning, the capsule is just in a little straight pile on the floor. (00:03:15 - 01:09:30)
Continuity mistake: The guy states that the room is sealed and his facial expression is one of panic. Camera cuts and now he's facing a different way and his expression has changed completely. (00:05:20)
Continuity mistake: When the elevator starts to drop from floor 10, the floor indicator says 7, but a moment later it counts down from 9. (00:06:05)
Continuity mistake: When the Red Queen kills all the workers in the main office, the positions and actions of people differ from the video footage to the actual events. (00:06:35)
Continuity mistake: When Alice wakes up in the shower, in one shot, you can see the shower curtain is not hanging over the edge of the shower. But when it cuts, it is. (00:08:40)
Continuity mistake: In the beginning when Alice is wiping the mirror, you can see her mouth is open. When it cuts back to her it is closed. (00:09:00)
Continuity mistake: Alice's scar on her left shoulder changes shape and redness throughout the film. (00:09:05 - 01:25:00)
Continuity mistake: Near the beginning of the movie, Milla Jovovich reads a note that has been left on the desk. She didn't write it, as they demonstrate in the film. However, when they do show the person who wrote the note composing it, the handwriting is different from that in the note from the earlier scene. (00:10:20 - 01:12:25)
Continuity mistake: In the scene where Matt pulls Alice into the mansion at the beginning of the movie and the SWAT team comes through the windows, the main SWAT member pulls Alice aside and asks for her report. If you watch the scene Alice has one arm at her side and one across her chest. A split second later it shows her reflected in the eye lenses on the SWAT member's mask and Alice has her arms crossed in front of her. Another split second later they show Alice again and her arms are in the original position. (00:13:35)
Continuity mistake: When Rain is under the train using her flashlight to see if she can see something the hair hanging across her face changes position between the wideshot and the close-up shot. (00:16:00)
Continuity mistake: When Rain tries the door on the train and fails, we see her face and her hair has 2 strands on one side of her face. Camera changes and now she's got 1 strand. (00:16:50)
Continuity mistake: On the train, just after Spence falls in, Alice takes off her wedding ring and sees on the inside: Property of Umbrella Corporation. However, after she finishes off Spence, she drops the ring next to him, and in that shot, the inside is clean; or at least the half we can see when it lies still. Such a long line would take up a lot of room on such a small ring in that size text. (00:17:45 - 01:19:05)
Continuity mistake: When the SWAT team arrives at the hive, the open an elevator shaft and one of them lights a flare with 2 hands. As the camera changes, he is now holding it at arm's length and with one hand without enough time to do so. (00:23:05)
Continuity mistake: When we see a CGI map of The Hive right before the Laser Room, the 3D thermal camera reveals a very rigidly stood up human body looking through the door's small window. When the camera cuts and we actually see Alice look through the window, she's slightly bent down so her head is on window level since she's taller. It's quite possible the CGI team just didn't spend more time than necessary to move the thermal human model's limbs and kept it in an almost default T-stance position. (00:29:30)
Continuity mistake: In the laser corridor, the team leader is stood upright when the lights comes on, but as the camera changes to a front view, he is crouched. (00:30:10)
Suggested correction: It's easy to miss, but there is a quick insert-shot in-between these two shots that shows the team leader crouching down suddenly. (The camera is focused on the back of his head as he ducks down suddenly.) It can be seen in this video (https://www.youtube.com/watch?v=rOLe15-5Xhw&t=65s) around the 1-minute mark.
Continuity mistake: When they are entering the entrance to the Queen's chamber, the Medic is carrying a bag as you can see when the door closes. When it cuts from inside the entrance the bag has disappeared. (00:30:50)
Continuity mistake: When the first laser passes through it hits the one guy in the wrist, however we see him on the floor missing half of his fingers and hand instead. (00:31:30)
