Trivia: When the first scientist was being evacuated by Umbrella's men in black, you can see on the background on her TV the commercial for "Umbrella's regenerate" commercial that was in the teaser trailer.
Trivia: As part of a promotion for this film, a mock-newspaper was distributed that had an article on Alice, revealing "Alice" to only be an alias and her real name to be "Janus Prospero." While this newspaper is no longer canonical to the films due to conflicting information with other movies in the series, at one point the third movie "Extinction" was going to reveal that Alice's real name was indeed "Janus Prospero", just as the promotional material for this movie hinted.
Trivia: Ben Moody, former lead-guitarist of the band Evanescence, has a cameo as a zombie in the film.
Trivia: Writer Paul WS Anderson had previously worked on the film adaptation of the video-game "Mortal Kombat", and included a small reference to the game/film during the climax. When Alice and Nemesis are fighting, at one point the main villain tells Alice to "Finish him!" This line is used repeatedly in both the video-game and film adaptation of "Mortal Kombat."