Continuity mistake: Apollo's lightning bolt throws Scotty across the table and dislodges a big bunch of green grapes from the fruit bowl. They fall onto the table. But when Kirk's crew later assemble at the table, the green grapes aren't there, or in the bowl, or anywhere in the temple. They magically reappear in the bowl just after Spock gets through to Kirk on the communicator. (00:18:55 - 00:41:10)
Continuity mistake: Mad Lazarus has just put a black-and-white hatch cover over the stolen dilithium crystal in his time ship. It's in place when Kirk arrives to confront him. But a moment later, when Kirk leans in and is accidentally transported to the negative universe, the cover has disappeared. It's still missing when Kirk returns to push Lazarus through the portal. (00:39:20)
Continuity mistake: When Kirk splits up the landing party, he asks McCoy to further inspect the vegetation. In the two shot, McCoy isn't wearing his tricorder, but in close-up, it's there, strapped over his left shoulder. Cut back to the two shot, though, and it's gone again. (00:10:20)
Continuity mistake: Kirk's arrow strikes the Klingon in the knee. But a few shots later, the shaft is protruding from his thigh, several inches above its original position. Still later, he's holding one hand to his calf just below the knee, which isn't where the arrow was either time. (00:43:40)
Continuity mistake: During the scene when the Klingon agent is exposed by the Tribbles, Kirk first holds the two furballs as if they had a handle on the underside (probably for the parts to make them wriggle). As he turns to Spock (the wide-angle shot), he holds the Tribbles cupped in his hands, his thumbs supporting their flanks. But as Kirk approaches Spock (close-up), he has them 'by the handle' again.
Continuity mistake: When Uhura tells Kirk that she's made contact with his private transmitter she's seated at the communications console, with the two overhead screens above her station, beside the turbo lift alcove. Yet after the contact's been broken, when she tells Kirk, "It's impossible at the moment because they stopped broadcasting," in this unusual shot facing Uhura the turbo lift alcove is not behind her - Uhura is actually seated much closer to Spock's station, note the upper section with overhead screens. Then she's properly seated at her station once again.
Continuity mistake: While Flavius guides Kirk, Spock, and McCoy to the city to find Merik, the foursome stop behind a group of trees. In the first wide shot, as Flavius explains they'll wait until dark, there are no red marks on the tree trunks, but in their closeups there are a few red marks (presumably from squibs), before the gunfire has even occurred. At the start of the second wide shot the tree trunks are still void of those marks, and when the police shoot at them that's when the red marks appear. (00:15:15)
Continuity mistake: When McCoy places the old tricycle down on the ground, the handlebars are facing the right with its wheels facing the left. When McCoy is attacked by the diseased Only, Kirk and Spock subdue him, and as he lies on the ground the tricycle flips over between shots, so the handlebars suddenly end up facing the left. (00:04:30)
Continuity mistake: When McCoy escorts Spock to the shuttlecraft hangar McCoy is wearing his short-sleeved medical tunic, but when Spock suggests that the doctor could wish him luck, in the next closeup McCoy is wearing his long-sleeved uniform. (00:29:40)