Deliberate mistake: When Bart is in the genius school, the teacher asks him if he knows what happens if he adds an acid to a base. He replies yes, adds them anyway and the lab explodes and a green goo comes out, turning Bart green. If you add an acid to a base, you just get salt and water.
Continuity mistake: While Bart starts the I.Q. quiz and is reading it out loud, his teacher says "Shh. Visualize it Bart." While this happens if you look in the background you can see the alphabet line. It starts out Tt Uu Vv Ww, then Bart's teacher blocks out some, then you see the letter T again.
Continuity mistake: At lunch, two boys exchange Bart's sandwich for a grape. When they give him the grape, it lands in Bart's right hand but in the next shot he has it in his left hand, and when one boy gives Bart a cup, the grape is back in his right hand again, and in the shot after that, the two items have changed positions.