Continuity mistake: During Andy's daydream at the beginning of the film, Mr. Potato Head has an eye patch over his left eye. When the daydream cuts back to reality we see that Andy has removed Mr. Potato Head's left eye to symbolize the eye patch. But when Andy's mum comes in with the video camera, his left eye is suddenly back in. (00:01:15 - 00:05:25)
THGhostContinuity mistake: When Woody and Co. accidentally configure Buzz to his Spanish setting, they remove two small screws from his back that fall onto the floor. But once Ham starts reading from the instruction manual, one of the screws that landed next to him has disappeared.
THGhost
Continuity mistake: When Mom gives Molly the donation box she writes "Sunnyside" on one side of the corrugated box. When the Sunnyside box is in the car and also when Mom carries it to the daycare, there is printed writing at the side of the box (to the right of the "e" in Sunnyside) under the hand hole, but when Bonnie's Mom carries the box into the butterfly room that writing has vanished, then it reappears when the kids go to recess. (00:19:25)
Super Grover
Continuity mistake: In the scene where Woody is escaping from the daycare for the first time, we see him in a hallway hiding behind a no running yellow stand up sign. He is also at the hallway corner because we can see two women talking down the other side of the corner. He then proceeds down the hallway a couple of steps, he then seeing the janitor coming and immediately falls very close to that sign, Then from a bird eye view scene next, woody is now in the middle off the hallways and the sign is nowhere to be found as the janitor rolls over woody where the sign should be.
Continuity mistake: Lotso throws Woody's hat in front of Jessie's box, which lands directly in front of her. In the next wide shot, the hat is still in front of her, but also to her right. The hat was not touched between shots.
bobthedancingdonutContinuity mistake: In the scene where the janitor goes into the bathroom to wash his hands, he originally, whilst entering the room pushing his cart complete in front of the stall, but when it cuts over to where woody sneaks into the stall, the cart is now moved to only halfway blocking the stall door.
Continuity mistake: When Woody and Buzz are standing on top of the cabinet looking at the photo they hear the kids and mom coming, they immediately jump down from the cabinet and land on the bottom open drawer, next to blue skateboard, then on the floor and they both run to the toy chest without looking back. Two shots later, when the door opens and the kids/mom come in, you can see that the drawer is now closed. (00:11:40)

Continuity mistake: After Chuckles tells the story of Lotso we see the gang locked up at the daycare center. Jessie comforts Bullseye and looks at the word "Andy" on her boot (with the "A" on the inner side) . Woody returns and when the bell rings for the kids to go outside, we see Jessie on the floor. Notice that the "Andy" on her boot is now different (the "A" is on the outer part of her boot). (00:52:50 - 00:57:10)
Continuity mistake: The bin lorry disappears instantly. The road is too long for it to have driven that entire length.
Ssiscool
Continuity mistake: When Mr. Potatohead has a tortilla for a body he has his eyebrows with him, but his eyebrows can't be detachable - there's no hole in his body for them to plug into, and they're not connected to his eyes.
Joel SheffieldContinuity mistake: When the toys escape from the daycare, the paint on their bodies appear and disappear back and forth.

Continuity mistake: When Bonnie gets the burger out of the microwave the door is left wide open. As the shot changes the door is shut. (00:34:10)
Ssiscool
Continuity mistake: When Woody is escaping through the bathroom stall window, he climbs up the stall on the same side as the janitor's right shoulder, but he climbs out in the mirror image on the janitor's left shoulder. You can also see the smudge is on his left shoulder. He escaped on the opposite side of the stall to which he climbed up.
Suggested correction: Please rewatch this scene. There are two stalls, one with the door closed and one with the door open, which is the stall with the window and Woody. There are also two shots where Woody has his foot stretched across to the window (sill). The first shot in the POV from behind the sink, is facing the janitor and Woody. The second shot is from behind the janitor's back, facing the mirror, and this shot zooms in at an angle to show the reflection of Woody and then the mirror's smudge. Woody is positioned correctly and standing in the proper place in both shots.
Super Grover
Continuity mistake: When the Fisher Price phone is telling Woody about the monkey, he also says the wall is eight feet tall. Security camera footage shows toys scaling the wall, almost making it to the top. When it cuts to the toys climbing, they are only about one or two feet off the ground.
bobthedancingdonutContinuity mistake: When the toys are put into the boxes on the shelves, Lotso steps up on some bricks to talk. A few shots later, a truck, which was not there before, takes Lotso and the other toys away.
bobthedancingdonut