Trivia: The directors have previously worked on both Community and Arrested Development - both shows get nods in this movie. Jim Rash, the Dean from Community, plays an MIT faculty member at the start. More subtle is the Bluth family's stair-car from Arrested Development, seen briefly as Captain America walks onto the airport runway before the big battle with the other Avengers.
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Trivia: In the comics Tony Stark was inspired to support the Superhuman Registration Act after an encounter by a woman named Miriam Sharpe. Miriam blamed Stark for the death of her son, who had died during the explosion in Sokovia (in age of Ultron in MCU) which opened Stark's eyes up to how reckless he and the other heroes had been.
Trivia: After Tony blows off Bucky's arm, Cap picks up his shield and shields himself from Tony's blasts. At the very end of the shot, Tony is firing a beam at Cap which lands on Cap's shield and the shot begins to slow down. This moment from the shot is an homage to the cover from part 7 of the Marvel Civil War comic storyline, where Captain America and Iron Man are fighting on the front cover in the same way.
Trivia: In the comics Tony Stark and Reed Richards worked to design a superhuman prison known as Prison 42, which was made specifically for super humans who disobeyed the Registration Act. It was top secret and was used to hold many of those on Team Cap about halfway through the arc. Captain America later broke his teammates out of the prison.
Trivia: Sharon Carter says a eulogy at her aunt Peggy's funeral, with the quote from Peggy, "your job is to plant yourself like a tree and say no, you move." This line is actually an iconic quote from the newer Captain America comics, and is said by Captain America, not Peggy Carter. The quote was said in Amazing Spider-Man #237, published in 2007.