Trivia: Alan Ritchson, who portrays Rafael in this film and the sequel "Out of the Shadows," has stated that the actors portraying the four turtles were mistreated on-set and by the studio. As they were technically portraying animated characters through motion capture, their contracts were reportedly "full of loopholes" that didn't afford them equal treatment with the rest of the live-action cast, despite them being on-set full-time doing grueling work in motion-capture suits. One particular instant included the four turtle actors being kicked off set at the end of a 14-hour day without transportation home, while the rest of the cast and crew were given rides. They also weren't paid overtime like the rest of the crew, were barred from giving interviews despite portraying the titular characters, weren't invited to the premier, and were subsequently denied back-end profit shares from the sequel that they had been promised.
Trivia: During Lucy's final transformation sequence, her consciousness starts traveling through time by millions of years. At one point, Lucy pauses in time to observe and interact with an ape-like creature, reaching out to touch its fingertip. Although not expressly stated or explained in the film, this ape-like creature was what scientists today classify as Australopithecus afarensis, the earliest identifiable ancestor of humankind. The first fossils of Australopithecus afarensis were determined to be from a female who was christened "Lucy" by scientists.
Trivia: The profuse drooling that Wallace suffers once he has been "transformed" into the walrus could not be helped - actor Justin Long was constantly drooling due to the heavy prosthetic makeup, and the fact he had to hold his tongue back to simulate the fact that his character's tongue had been cut out.
Trivia: When the mayor is giving his presentation about the plans for new development, one of the men is fiddling with a tiny black model car. He causes it to pop a wheelie and cruise down the model street; just like the black Dodge Charger Vin Diesel popped a wheelie in during his climactic race with Paul Walker, who also stars in this movie, in The Fast and the Furious.