Character mistake: Seven minutes into the show, Sarah Sidle is about to do an internal sexual assault exam/kit on an unconscious victim in the hospital, Sarah picks up a metal speculum and says aloud to the victim (in a presumed moment of empathy), that she "never really liked this part of my yearly exam. These things are always freezing" referring to the speculum in her hands. She then brings a speculum to her mouth and begins to blow open-mouthed on it two times, forcing her hot breath on it to warm it. She then begins to insert it into the victim as the scene cuts away. This is pure stupidity, as no trained CSI would ever contaminate the tool like this. Sarah just added her own DNA to the speculum via her breath so any saliva or body fluids are now on the speculum what she is about to use on this patient, who is now also exposed to any STDs from Sarah.
Continuity mistake: When Sara is showing Greg the route the old woman took in her car, she draws a blue line on the screen with her finger. When she says something about turning left she draws a continuous line to the "Meadows Lane," but when the shot changes and goes back the line is broken into two.
Continuity mistake: Sara takes pictures of Brenda with an UV light camera. The camera used to film the episode for broadcast is barely able to show the walls in the background as tiled. When the UV pictures of Brenda are used in the interrogation, the photos show Brenda's back up against the tile wall.