Corrected entry: During his fight with the slayer during the Boxer Rebellion, Spike is cut on his left eyebrow. This was done to give a reason for James Marsters' real scar, but vampires can regenerate. Later in the season, Spike is tortured and beaten by Glory without a scar to show for it after he healed. For that scar to be there, Spike would have to be cut before he was turned into a vampire.
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