Continuity mistake: When Ash takes his testing number, his gloves do not cover his thumbs, but in the next shot, they do. (00:02:58)
Continuity mistake: When Ash tells Todd to save his photography for Pokémon, Todd's blue camera case turns black, while the strap turns yellow, like the one on his camera. (00:04:33)
Continuity mistake: When Meowth is suspicious of Jessie, pads appear on his forepaws. (00:06:11)
Continuity mistake: When Jessie says the swirl is an Arbok, her blue eyes turn green. In the same shot, her hair is not fully coloured in and the background can be seen through it. (00:08:37)
Continuity mistake: When Ash and Team Rocket are surprised, the instructor reveals the answer to the swirl question. Ash's right shoe changes from black to white. (00:08:46)
Continuity mistake: As the scoreboard lowers, Todd has his bags with him, but when he asks where Ash's picture is, they disappear. (00:10:07)
Continuity mistake: When Ash tells Weezing to use Smog, the left sleeve on Ash's jacket changes from white to blue. (00:14:12)
Continuity mistake: When the instructor sends out Jolteon and Vaporeon, the lower part of his shirt collar changes from blue to red. (00:14:51 - 00:16:12)
Continuity mistake: When the instructor tells Jolteon to use Agility, the sides of his shirt collar change from red to blue. (00:15:11)
Continuity mistake: When Ash recalls Arbok, its Poké Ball is upright, but in the next shot, it is upside-down. (00:15:50)
Continuity mistake: When Jessie says there will not be a next time for the instructor, and when the Pokémon turn against Team Rocket, the inside of Ivysaur's ears changes from black to green. (00:17:42 - 00:18:10)
Answer: Yes it ran away because Layla hurt its feelings.