Plot hole: Lucy jumps from the airliner and flies her hang-glider straight to Eduardo/El Macho's Cinco de Mayo party to find Gru. How did she know Gru would be there? He had no intention of going and only did so after he was pestered into it by the girls on the very night of the party, so he couldn't have told her himself. Shouldn't she have gone to his house instead? (01:09:50)
Continuity mistake: In the scene where Gru first meets Lucy Wilde, Gru takes out his freeze ray and fires it at Lucy. Lucy stops the freeze with a flame gun thing and then she walks over to him and tazes him. Gru then drops the freeze ray, but it disappears when she goes to put Gru in the boot of her car. (00:08:30)
Continuity mistake: When Tom the Minion dressed as a maid is hoovering, he answers the front door by opening it towards him as does the minion who looks out of the door after Tom has been taken. In the scene with Gru sat on the steps in the rain, sad that Lucy is off to Australia, Agnes comes out with an umbrella, but now the door opens outwards.
Continuity mistake: After watching El macho activate the dance floor key to the lift, Gru then does the same thing. During the first attempt, Gru spills guacamole on the floor, his nacho hat gets sliced in half and more guacamole falls. He then throws the nacho hat to one side and tries again. But in the shot from above the guacamole on the floor has vanished. (01:04:10)
Continuity mistake: When Gru is attempting to unlock the floor key pad, he triggers an incorrect key and some fire is blown at him. Some smoke is left all over his face. Gru triggers another incorrect key and some darts are launched at him. The smoke is still there, but as he falls over, most of the smoke on his face has gone. The smoke on his right cheek is still there, but has still vanished from his face. (01:04:20)
Plot hole: The rocket is launched with Gru and Lucy attached to it. All of the minions are around them at El Macho's lair at this point. Considering a rocket travels at several hundred miles per hour, when they jump off it almost a minute later they should be several miles away at the very least, but three Minions appear on a boat shortly after. There is no way the three Minions could have reached Gru and Lucy all the way from the lair in that short time. (01:22:05 - 01:23:25)
Audio problem: As Gru attempts the dance floor key, some of the guacamole from his nacho hat falls out of the hat and lands on one of the keys which then triggers one of the sound effects. An axe then chops off the rear end of his nacho hat and lands on the part of the dance floor behind him, but no sound effect is made from the floor. (01:04:05)
Continuity mistake: When Gru follows Eduardo through the corridors, he stumbles to find him dancing along a floor key pad to unlock the lift. It is also shown that if a key is pushed incorrectly, the key turns red and a weapon is launched at the person on the key pad. When he begins to activate the key pad, the first key he steps on is the key second to the left on the first row and continues the rest of the pattern. Gru then attempts to do the exact same sequence. The first key he steps on is the key second to the left on the second row, despite the fact that Eduardo actually triggered the first sound effect with the key on the first row, second to the left. Despite this, the sound effect is still correctly triggered. (01:03:40)
Continuity mistake: One of the minions is shown dressed as a maid hoovering the floor. The hoover continues to move along the floor after the minion answers the door. The hoover is shown moving past the door. But when the next minion is shown, the hoover is now shown only just going past the door. (00:21:45)
Other mistake: When Agnes screams after the evil minion tries to eat her unicorn, the noise shatters glass items and a sculpture on the mantelpiece as well as the evil minion's goggles. But Margo's glasses do not appear shattered at all.
Other mistake: As Eduardo goes to leave the cupcake shop, Gru believes he could be El Macho and Eduardo leaves. Gru tells Lucy about El Macho for about 90 seconds, then Eduardo is seen from outside the cake shop window only just about to go up the escalator, when the distance between the shop and escalator is only a few meters. (00:27:55 - 00:29:25)