Continuity mistake: When Ash says, "We gotta make sure we don't get separated," Misty's right arm is violet, but in the next shot, it is skin-colour. (00:01:32)
Audio problem: When Ash says, "Up here," his mouth does not move. Also, a part of Pikachu's tail is mistakenly coloured yellow instead of brown occasionally. (00:01:42)
Continuity mistake: When Misty asks if Brock knows how scared it makes her feel when he yells, the purple strap on her bag turns yellow like her shirt. (00:02:04)
Other mistake: When Ash and friends are first seen when a Ghost Pokémon laughs, the base on Pikachu's tail is missing. (00:07:47)
Continuity mistake: When Brock says if there are Ghost-type Pokémon, then they can show themselves to them, he, Ash, and Misty are between a doorway, but in the next shot, they are not. (00:08:33)
Continuity mistake: When Ash asks how they would succeed in their match at the Saffron City Gym if they do not go back into the Pokémon Tower, Charmander is on a connecting part of the path, but in the next shot, he is not. Also, Pikachu is on one completely, but in the next shot, he is not. (00:10:31)
Answer: Yes it ran away because Layla hurt its feelings.