Continuity mistake: Tony Trihull appears when Finn McMissile begins chasing Professor Z. It also shows when Tony uses his magnet. When it is used, Finn's equipment are pulled by the magnet, which includes his bullets. Those bullets are black when all of them were going out. However, when they are pulled onto the magnet they are grey.
Continuity mistake: When the cars are zigzagging in the Tokyo track, there is a shot where three specialists are talking about McQueen and they show him on the track in split screens (air and ground shot). In the air shot, he has a red car behind and then a green one following. But in the ground shot, the red car is gone and the green is behind McQueen, even though you can see the red one in the air shot at the same time.
Continuity mistake: When the other tow truck asks how Holly Shiftwell's grandfather is, you can see that his Ivan symbol has a red background, and then a few minutes later you can see that Mater's Ivan symbol is white, and a few minutes later there is no background at all on Mater's symbol.
Other mistake: In the scene in the restaurant, where Lightning McQueen just hung up with Francesco, Sally says "You never bring him to any of your races" as a reference to Mater, even though Mater was at McQueen's last race in the original.