Continuity mistake: Mario initially has a tie wrapped around his neck in the scene where the Chipmunks and Chipettes are being forced into Klaus and Claudia's Rolls Royce. It's also worth mentioning that Mario's hands are tied behind his back. Pretty soon the car swerves head-on into a pole. In the following shot we see Klaus, Claudia and Mario lying on the floor disoriented - but now he's sporting a bow tie instead. (01:07:50 - 01:09:40)
Continuity mistake: When Eleanor is trying to feed the ailing baby penguin with a spoon, it refuses to eat. So she places the spoon near two bowls at her side. In the following shot Eleanor can be seen dipping a rag into one of the bowls - but now her spoon is nowhere to be found. (00:54:00)
Continuity mistake: When Miss Miller is pulling items of food from a cabinet, the Chipmunks are busy having breakfast. There is a carton of milk on the table right between Alvin and Simon's bowls of cereal. But after a shot of Theodore indulging in his meal, the milk carton is gone. Now Alvin and Simon have two glasses of milk instead, and who knows how they got there because Miss Miller was too busy talking to herself on the other end of the kitchen. (00:11:50)
Continuity mistake: When the Chipmunks and Chipettes are approaching an airport in their hot air balloon, Simon reaches over the side and shouts, "We're home!" Notice how he and Alvin are wearing regular long-sleeve blue and red shirts respectively. But in the next shot when Dave's plane draws near, they're now wearing exotic clothes from their trip around the world instead. (01:05:35)
Continuity mistake: Right before each balloon takes off for the race, Sophie is seen gnawing on a rope and behind her - on the grass next to the Chipettes' basket - a couple of wooden crates are visible. But in the following shot where the teams are in midair struggling to maneuver their balloons, no crates can be seen anywhere on the ground below. (00:15:35)
Continuity mistake: When the Chipmunks are delivering a Chipmunk doll in Venice, Simon holds a Simon doll in the long shot. When the cap of the post in the water is lifted, Simon inserts an Alvin doll.
Continuity mistake: After trying to entice the primitive tribe with flashy clothes and a boom box, Alvin and Simon get tied to a hut. The four ropes around their wrists are initially tied together in one knot right above their heads. But once they notice that Theodore is being pampered like a god, Alvin and Simon's wrist ropes are suddenly knotted separately instead. (00:51:05)
Continuity mistake: When Clause and Claudia are pushing Alvin and the rest into their car at the end, Alvin is seen without his cap. You then see a shot of the inside of their car, and in the next shot Alvin's cap magically appears on his head and blows away. His cap is still off until after he hugs David at the end, then it's back on his head again.
Continuity mistake: In the final scene where Miss Miller is sitting next to Theodore adjusting her hair in the car's busted rear-view mirror, the shot changes to Dave opening the passenger's side door. But as he steps in, Miss Miller skids away and Theodore can now be seen sitting with the other Chipmunks and Chipettes in the back seat. (01:11:30)
Continuity mistake: On their way to the Parthenon, the Chipmunks knock down a fruit vendor's tent. When Dave appears from beneath the sheet a second later we can see how one of the tent's support poles is broken in half, but in the previous shot none of the poles were even close to being damaged as they toppled over. (00:34:40)