Continuity mistake: Shortly after Dr Scott crashes through the wall and confronts Frank, their conversation leads to Eddie's whereabouts. When Dr Scott tells Frank, 'I can assure you that Brad's presence comes as a complete surprise to me,' he is leaning forward in his wheelchair. In the next shot, when he says, 'I came here to find Eddie' he is leaning back in his wheelchair.
Plot hole: Before "Rose Tint My World" the nude statues of Brad, Janet, Dr. Scott, Columbia, and Rocky are in the same positions, but somehow Frank-N-Furter managed to dress them despite the following impossibilities: 1) All are wearing high-heels despite being frozen while their feet are flat on the ground. 2) The fingerless gloves have a loop through which their index finger protrudes, despite their solid stone fists, except Janet whose stone fingers are solidly entangled in her stone hair. 3) All of Dr. Scott's clothes have been put on him despite being attached to the stone wheelchair and his legs covered with the stone blanket. (01:12:35 - 01:16:30)
Revealing mistake: At the end of the movie when the castle shoots into space, if you look just as it gets really misty, in the background (it's maybe just centimetres off) you can see the real castle. The one that blasts off is a cutout. (01:34:10)
Visible crew/equipment: Near the end of the "rose tint my world" performance, after Dr Scott wheels off stage, you can see a crew member with flares on, walking up in the rigging above. (01:21:05)
Deliberate mistake: In order to create the effect where Brad, Janet and Dr. Scott's bodies are turned into stone by Frank, casts were made of the actor's bodies, to create statues of them for the scene. The device turns their bodies into stone, but somehow a pedestal appears beneath both Brad and Janet - although this is obviously to make the statutes stand, where would the pedestals actually appear from in the story? Richard O'Brien, the screenwriter and writer of the original musical, referenced to the pedestals during the DVD commentary, expressing his dislike for them, but also questioning where they would have appeared from.
Revealing mistake: At the end of "Hot Patootie," an enraged Frank-N-Furter snatches an ice axe from the deep-freezer and menaces the terrified Eddie. There is no blood seen in the freezer at this point. Frank swings his first blow and completely misses Eddie, who is scampering into the deep-freeze several feet ahead of Frank. There is no blood seen in the freezer at this point, either. Frank then pursues Eddie into the fog at the back of the deep-freezer and viciously murders him with the ice axe (a genuinely horrifying scene as Tim Curry plays it, but the murder occurs entirely off-camera). But then, as Frank emerges from the freezer moments later, we see a splattered and gory trail of fresh blood leading into the freezer, as though Frank had already mortally wounded the escaping Eddie even before the offscreen murder. This looks as though footage from an alternate and more graphically violent version of this scene was kept in the revised scene, causing the gory blood trail to appear out of nowhere. (00:45:15)
Continuity mistake: During the Cabaret show, when everybody's stone bodies are placed on the stage, Rocky's right arm is extended forward and he has red make-up on his eyes. After he is unfrozen, he moves his right hand away from his forehead and the make-up on his eyes is now blue.