Continuity mistake: The dog catcher looks down at his gas gauge and sees that it's empty, and it's bright daylight. Then in the next shot he's pulling into a gas station and it's night time. Either bad continuity or if nothing else he managed to keep driving until night time on an empty tank.
Plot hole: At the start of the movie, a great deal is made of the boom mic that enters the shot. This is ridiculous, because Bolt's view would not be restricted to what was onscreen, he'd have a perfect view of the person holding the boom mic (as evidenced by it not being stable). There's no possibility of him not seeing the person holding the mic either. As Bolt completely and utterly believes the world he lives in, and thinks the situations he finds himself in (saving Penny) are real, someone holding a boom mic would break the illusion for him. In all instances in the movie where an outside force (i.e. a dog handler) interacts with him, Bolt never gets to see the person handling him (handler approaches from behind), the boom mic operator however is literally right in front of him.
Suggested correction: He wasn't paying attention and considering that his focus was only on the "action", how the hell would he notice a boom mic when he's trying to protect Penny? His "reality" is never broken even AFTER he's in his trailer.
Factual error: Near the beginning of the film, Calico is sitting with a cat on his right shoulder. Their reflection is shown in the glasses of a man directly opposite. The reflection shows Calico and the cat in the same position instead of being reversed, as a reflection would be. (00:04:30)
Deliberate mistake: When Penny is loaded onto the ambulance at the end, her gurney is rolled straight in. This is wrong, the paramedics will collapse the wheels and slide the gurney into locks to hold it still during the drive. It was done this way since Mittens and Rhino were hiding in the collapsible section.
Continuity mistake: At the beginning, when Penny and Bolt are acting out the chase scene on the playback monitor, the producers are enraged over a boom mic showing up in the shot. Yet seconds earlier during the actual scene, you don't see the boom mic in the upper right corner. (00:10:10 - 00:11:50)
Continuity mistake: In the beginning, at the bridge scene, the man in the car's sunglasses fall from his face and the wind seemingly directs them to the boat underneath. The shot changes to Penny, and her hair is being blown the opposite way.
Continuity mistake: After jumping off the back of the moving van, Mittens brushes a twig and leaf off of herself - both appearing to land beside her. But when she threatens Bolt with the styrofoam, the twig and leaf are gone.
Factual error: When Bolt is in New York City, we can see a pedestrian "WALK/DON'T WALK" sign. In New York City, these kind of signs are a white walking character and a red hand indicating to stop.
Deliberate mistake: When the song comes on showing the three travelling across the U.S. on the map, it has them in the bed of the dumptruck, on top of a dirt pile going across 3 states. A dumptruck wouldn't be travelling that far with a load of dirt that can be gotten in any state.
Continuity mistake: Towards the end of the film when Penny takes one last picture, Mittens is lying against the mother's lap. But at the very end when you see the photo, Mittens is sitting up and posing.
Continuity mistake: When the man hears barks coming from the shipping box Bolt is in, he takes a knife and slices a line through the tape that goes across the top of the box. Then the next shot shows all the tape sliced. (00:26:00)
Continuity mistake: When they discover the boom mic in the background, there are two monitors in the background. Both seem to have the same time and date on them, yet they show Bolt in completely different positions.
Continuity mistake: When Penny and Bolt are doing their show at the beginning, Penny is on her scooter when she tells Bolt to "speak" and also when the shot comes up when the camera is right in front of Bolt's face(right before the superbark). But when cam 5 comes up where the man is talking to Mindy Parker about Bolt the scooter is laying on the ground and Penny is not on it.
Continuity mistake: Penny takes a photo of herself with Bolt after Bolt has 'rescued' her again at the very beginning with a Polaroid camera. She places it (the camera) on a counter next to the trailer's door and it is parallel to the edge. Later, after the two cats have taunted Bolt and the camera pans down, the camera has moved and is now positioned at an angle on the counter.
Continuity mistake: When Penny is on her scooter and Bolt has a bomb with a timer on it reading 34 seconds, it actually closer to 50 seconds before bomb detonates.
Continuity mistake: Penny pours dog food onto a bowl for Bolt and places it next a pillow. Cut to Penny trying to get Bolt to play with his toys and a bone design has appeared on the bowl. It's too fast for Penny to turn the bowl around. (00:16:35)