Continuity mistake: In the scene at school when Gwen invites Peter to have dinner at her house, Peter initially removes both earphones when Gwen talks to him, but when Gwen asks about the bruise in Peter's eye and the camera angle shifts, the right earphone is still in his ear.
Continuity mistake: When the one of the cups of coffee falls off the table Peter catches it. After that, Peter takes the other cup from the desk to give Dr. Connors. In the first shot Peter carries the cup from the upper part, next shot the cup is on his palm.
Continuity mistake: When Peter is looking through his father's file in his bedroom, Uncle Ben comes in and starts playing with a Rubik's cube from Peter's desk. When he sets it down, the top and middle row are red and the bottom row is white. Shortly afterwards, when Peter is researching Doctor Connors on his computer, the top and middle rows are suddenly white and the bottom row is red (not fully solved, as another listed mistake claims).
Continuity mistake: In the scene when Peter Parker goes back to his Aunt & Uncles house after his first Spidey-training session, he raids the fridge & freezer. When confronted by his Aunt & Uncle, the freezer door is slowly swinging open, but in the next shot, the freezer door next to his Aunt & Uncle is closed.
Continuity mistake: During the scene where Spider-Man is unmasked he splits an assault rifle in half, trips a man, then drops the two pieces on the ground. The scene switches to a long shot and Spider-Man can be seen still holding the two pieces of the weapon in his hands. The pieces are out of his hands in the immediate following shot. (01:44:50)
Continuity mistake: Just when Peter tries the web launcher the tables and chairs on the street change number and position through shots: They are arranged parallel to each other and have the following amount of chairs: First table has 3 chairs, second 2, third 1 and fourth 2. In the close-up, all tables have 2 chairs; the first three tables are parallel, yet a fourth one is behind the third table. Peter also knocks down a table or chair which appears standing in the final shot.