Continuity mistake: When Robert Neville sees Fred at the end of the road and is lured into the darkseeker's snare trap, before Neville actually gets caught and hung upside down (approaching Fred from the truck and shooting at him), the position of the sun in the sky, and angles of light in the scene, change dramatically in only a few minutes time.
Continuity mistake: At the end of the movie, when Nevile is behind the glass and he is thinking and all the sound goes away, you can see the bite mark that the zombie gave him. While the shot is changing between him and the zombie, you can see the bite mark change sides of his shirt.
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Continuity mistake: At the beginning of the movie when he is driving his Mustang, the movie is shot inside the car several times. If you look carefully you can see that the same background scene has been used twice. In the scene, you see a red wall from a building and a grey car standing along that red wall. This scene is used twice.
Continuity mistake: In the scene were Robert Neville is hanging upside down from the trap that he walked into, he starts to bleed at the back of his head after hitting his head on the floor. However, the next day when Robert shows Anna his Bob Marley CD, there's a shot of the back of Robert's head after he turns the volume up, an there is no cut, graze or wound visible.
00:46:40 - 01:14:20MichaelLFC