Factual error: In some of the scenes in the video during their time at Readville camp, they celebrate Christmas. In actuality, the first volunteers arrived at Readville in February of 1863, and Shaw died in July of 1863, along with most of the regiment, so there is no possibility of having a Christmas during that time.
Factual error: During the burial scene at the end of the movie, the corpses of the 54th soldiers are shown with only their shoes removed. In reality, the corpses of the 54th dead were stripped of all usable clothing, as the South was desparate for any uniform parts (source: "A Brave, Black Regiment" by Louis Emilio). At the very least, the corpses of the officers would have been picked clean by souvenir hunters.
Factual error: During the training of the regiment, there is one scene where Colonel Shaw is shown practising his swordsmanship. A row of stakes has been placed in the ground and a watermelon tied to each one which he slices apart with his sword as he gallops past.The caption tells us that it is February 1863 in Massachusetts. There is no way you could get fresh watermelons in that state at that time of year in 1863.
Factual error: One of the characters of Glory is a mute drummer boy. However, 54th Mass. was established as a regiment composed exclusively of the cream of Northern black society. Because only the most healthy and robust African-Americans were allowed to enlist in this regiment, many recruits were rejected for medical reasons. Union military officials of 54th Mass. would not have recruited any handicapped person.