Continuity mistake: During Santanico Pandemonium's routine, two dancers take the snake away so she can step forward. As they take the snake off, the second dancer is holding only a couple feet of the snake's tail, but in the next shot, the second dancer is holding the entire snake. (00:55:40)
Continuity mistake: In the hotel when the manager comes through the door, in the first shot the door is open, but the next time the door is closed.
Continuity mistake: Frost gets green blood all over his hand after holding the vampires heart, but this disappears while they are fighting the four women. After the fight finishes, the blood returns.
Continuity mistake: In the opening scene, the pages change in the magazine that the convenience store clerk is reading. (00:01:30)
Continuity mistake: The scene where the vampires in the Titty Twister bar start devouring the customers. George Clooney confronts Salma Hayek. She taunts him and he shoots her. When he does this the jacket falls open to show he is wearing a white shirt underneath. However, when she comes back to life a couple of seconds later and punches him to the ground, his jacket falls open again - only this time it is a completely black shirt underneath.