Corrected entry: When you first see the cable car (at the end) it says Universal Pictures. When you see the car the second time, it says Roosevelt Line.
Corrected entry: During the end sequence, Spider-Man originally did his final swing through the World Trade Center towers, landing on top of the tower with a flagpole on top. This was changed after September 11th so that he lands on the Empire State Building instead. However, during the pan around him after the landing, you can still see the Empire State Building, which he just landed on, in the background!
Corrected entry: When Peter gets out of the car when Ben gives him a lift, we see there is a bit of his costume sticking out under his shirt, but when we see him a second later, the costume can't be seen.
Corrected entry: At the beginning of the movie, when Peter is chasing after the school bus you can clearly see a bridge ahead of the bus. But neither the bus, nor Parker, go under the bridge.