Factual error: Hannibal makes a bomb using latex gloves that were not in use during the early 50's when the movie takes place. (01:28:35)
Continuity mistake: The amount of sacks by the oven that Laura moves away keeps increasing and decreasing randomly all the time.
Continuity mistake: When the car dies on the side of the road the headlights are off, but when Amy gets out of the car to go with David you catch the headlights shutting off.
Continuity mistake: Mike avoids colliding head-on with a pickup by swerving off the road into a farm field. He does this after passing a tractor and flatbed truck to his right. The shot changes and he is turning off the road into the field between the tractor and truck. (01:36:50)
Revealing mistake: On night 21 the time-stamp changes from 11:59:56 PM to 11:59:57 AM despite it not having turned midnight yet.
Continuity mistake: When the guy and girl are sitting in the car, they put the newspaper with duct tape up on the window but later on both the duct tape and newspaper are gone.
Continuity mistake: At the start, Tillman is reaching into the water with his right arm, as seen from the crocodile's perspective. But seen from above surface, he is reaching with his left arm.
Visible crew/equipment: When El Wray and the police arrive at the hospital as Dakota drives off, at the very back of the shot is the crane used for the lighting (confirmed on audio commentary).
Continuity mistake: Right after Jim has his nightmare while sleeping in the car, he looks around for Grace, who is standing outside the car looking at scenery. When Jim sees her, she has her arms crossed over her chest. The next shot, with Jim walking around the car, she has her arms by her side, and immediately after, the shot with Jim approaching her, she has her arms crossed again. All this took place in about 2 seconds, not enough time to drop her arms and recross them exactly the same way as the first shot. (00:19:35)
Continuity mistake: When Abe is being tortured by the Harpies, at one point, you can see that his body is covered with cuts and his blue-colored blood. The blood then disappears and reappears in subsequent shots.
Factual error: After the little girl is taken they play audio of the mother on the phone with 911, the operator says they can't do anything for 24 hours. Police do not wait 24 hours to look for a missing person, especially a child.
Visible crew/equipment: When the ambulance is about to crash into the tanker, right before and during the crash a camera mounted (painted white) on top of the cab is seen.
Factual error: When Tom shocks Angela with a stun gun, she gets knocked out. That's impossible. Stun guns don't knock you out. Stun guns don't cause unconsciousness.
Continuity mistake: The left tail light of Sadie's car is broken when she picks up the unfortunate hitchhiker. The light works when she turns left off the road. (00:36:20 - 00:38:35)
Revealing mistake: Sean Patrick Flanery plays a nerd who has trapped a female vampire in a cage. About 3/4 of the way into the movie, he tricks a co-worker into visiting, planning to feed him to her. He extends his right arm through the bars, and she starts to caress and kiss it, then begins to bite it and suck his blood. When he tries to withdraw, she tears off his arm at the shoulder. But in the next shot, he and Flannery both hit the floor outside the cage, and the co-worker's right arm is visible. However, when the scene begins, both Flanery and the co-worker are wearing watches on their left wrists, and in the shot of them on the floor, both have watches on the right, suggesting that the image has been reversed.
Continuity mistake: After hitting the animal in the forest when entering the park, the windscreen of the van is covered in blood. In the next shot the van is shiny and looks like it has just been washed, but a few seconds later it has dried blood on it again.
Continuity mistake: In the scene where Mackenzie's father is at the grill BBQing, you see a remote control in his shirt pocket. The remote control disappears and reappears in several shots.
Revealing mistake: After Steven Wilkins disguised as a vampire kills the unnamed woman he closes her eyelids to make it look like she's asleep. Just before his hand contacts her eyelids you see her almost blink, revealing she's very much alive.
Continuity mistake: When Nicole breaks the glowstick and splashes the liquid on the killer's mask, she starts to run. In the next shot of the killer, there is no liquid on the mask, yet it reappears in subsequent shots. (01:03:20)
Factual error: When the wraiths first invade the stockade, one of the officers orders his men to fire their "rifles". This is the 16th century. They're firing muskets.