Other mistake: On the "Mario's Greatest Movie Moments" DVD of this series, from the main menu, go into the trivia game. The fifth question asks you about the Super Mario Bros. Super Show episode 'Count Koopula.' It asks you "What's room number that the Mario Bros. were put into in Koopula's Castle?" It's supposed to say "What's THE room number that the Mario Bros. were put into in Koopula's Castle?"
Continuity mistake: This mistake is in the intro with Mario and Luigi in real life. When Mario breaks the ceiling while Bowser is holding a yellow bar, Mario's gloves disappear from his hands when he jumps to break the ceiling. When he lands, his gloves reappear back on his hands.