Continuity mistake: Near the start, when Ray is showing the photograph of Madeline with the security guard to passersby asking if they've seen her and also when he shows it to Nick, we can see the photo Ray is holding out in the wide shots, but in the two closeups of the photograph it's actually a different photo. Then later, when Ray shows the photo to Madeline's mother it's the same as the one from the closeup earlier.
Character mistake: Seven minutes into the show, Sarah Sidle is about to do an internal sexual assault exam/kit on an unconscious victim in the hospital, Sarah picks up a metal speculum and says aloud to the victim (in a presumed moment of empathy), that she "never really liked this part of my yearly exam. These things are always freezing" referring to the speculum in her hands. She then brings a speculum to her mouth and begins to blow open-mouthed on it two times, forcing her hot breath on it to warm it. She then begins to insert it into the victim as the scene cuts away. This is pure stupidity, as no trained CSI would ever contaminate the tool like this. Sarah just added her own DNA to the speculum via her breath so any saliva or body fluids are now on the speculum what she is about to use on this patient, who is now also exposed to any STDs from Sarah.
Trivia: For those who don't (or can't) read American Sign Language, at the end of the episode, Grissom says to Dr. Gilbet is that his mother lost her hearing when she was eight years old. He once asked her what is it like to be deaf, and she told Grissom (who loved to swim) that it was like being underwater. She also taught him that being deaf does not make one inferior to others. Dr. Gilbert then replies that she teaches her students the same lesson.
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