Cool Change - S1-E2
Plot hole: Grissom examines the victim's body and immediately rules it a homicide because he was wearing eyeglasses. He states that suicide is a cowardly act and no coward wants to see their death and would have removed their glasses before committing suicide. What a completely unfounded, and unscientific, statement. Suicide being an act of cowardice is his opinion and not a scientific fact and they don't work off opinions: they always state how they work off the evidence.
Suggested correction: This is correct, that was not scientific on Grissom's part, however this wouldn't be the last time Grissom formed an unscientific opinion that he was sure of based off what he thinks to he true as have the rest of the CSIs in several cases throughout the series. This also happens in real life; people involved in crime scenes making assumptions thus making errors off it. It wasn't right; but I would hardly call this a plot hole as this is something Grissom legitimately believes.