Continuity mistake: In the scene where Chicken Little lends the alien baby, Kirby, his handkerchief, after Kirby blows his nose, it sticks to Chicken Little's hand. Chicken Little then wipes the hanky off his hand on to the grass. In the very next shot, the hanky has disappeared, and never shows up again.
Continuity mistake: In the scene where Chicken Little brings his friends to see the panel in his bedroom, there is a pendant behind the pig's right shoulder. After Chicken Little says, "OK, subject change", the pendant is not there, but there's a door instead. There would be no room for the pendant to have been there in the first place.
Trivia: After the water tower ball goes through the movie theatre in the opening sequence, the ball then crushes three cars. Their horns sound off the M-O-U-S-E portion of the Mickey Mouse Club theme.