Plot hole: Property is destroyed, museum windows are crashed through, a giant octopus splashes about in the National Mall's reflecting pool, and even the statue of Honest Abe at the Lincoln Memorial comes alive. With all this occurring, not a single D.C. resident seems to notice and nary a single police officer turns up to investigate the goings-on.
Other mistake: When Larry first enters the main storage room, he has to go by the guard Booth. A voice says "ID" then acknowledges that it's "Brundon, later dude". When it shows Larry from behind it shows a clear shot of the inside of the entire security room. It is completely empty. Did a ghost ask for the ID?
Continuity mistake: In the scene where Larry and Amelia escape into the VJ Day photograph, Larry is seen speaking to a sailor in dress blues. Throughout the exchange the rating badge on the sailor's arm changes from crossed keys, the insignia for storekeeper to lightning bolts, the insignia for radioman several times.
Continuity mistake: When Ben Stiller is dropped off from the red plane in the middle of the street, the streetlights in the background are yellow for a moment, then switch to another angle and they are green (without adequate time to switch back), then they constantly switch between green and red depending on the camera angle.
Continuity mistake: When Jedediah is in the hour glass, he is in the lower chamber, and the area between is too small for him to go through. When the minions are returning to the portal, in a close up he is obviously in the top chamber, only to return to the bottom when Octavius breaks him free.