Continuity mistake: At the beginning of the second scene, you can see a man with a camera filming the penguins, but when one penguin says, "Smile and wave," the camera man has disappeared.
Other mistake: In the scene on the boat where the mute monkey is reading the notice on the penguin's box, and signing to the other, the monkey who can speak (but not read) is translating faster than the mute monkey is signing. For instance, he states the notice says "Kenya" before the mute monkey fingerspells "Kenya".
Factual error: In the sequence where the animals get on the first subway, it says they are on the 59th St subway. But, there is no 59th St subway in NY. The only subway station close to 59 is the 57th St subway.