Alfred: Could it be an old foe in a new disguise? Perhaps Catwoman?
Bruce Wayne: No. Even Selina Kyle has more regard for human life. This is someone entirely new, Alfred. The last thing Gotham City needs is some vigilante running amok.
Alfred: As they say in the streets, I ain't touching that one.
When the Batwoman is fleeing at the very beginning of the movie, she is shot at by her pursuers from below. The shot, represented by a yellow streak, is then seen coming from above before it strikes the vehicle she is on.