Trivia: When J. Jonah Jameson is thinking of a name for Octavius, Hoffman shouts out "Dr. Strange" and JJJ says "Already taken." Dr. Strange is another Marvel hero who lives in New York. Director Sam Raimi would later go on to direct Doctor Strange in the Multiverse of Madness after original director Scott Derrickson stepped down.
Trivia: In the scene where Peter drops his books on the college grounds and bends down to pick them up, a black bag comes by and smacks Peter in the face. The person carrying the bag is director Sam Raimi.
Trivia: In the hospital scene when Doc Ock's mechanical arms are attacking the surgeons, there is a scene where the camera cuts to a surgeon raising the chainsaw in the air. That scene looks a lot like the scene from "Evil Dead 2" (1993) where the camera does the same thing when Ash grabs the chainsaw to cut off his hand. Another Sam Raimi film.
Trivia: In one scene after Spider-Man has lost his powers, he catches a ride on an elevator. On one floor, a young man gets on and comments about Peter's "great Spidey costume". Peter says he made it himself, it itches, and rides up in the crotch, causing a strange look from the young man. The young man was Hal Sparks, who plays Michael Novotny on the US version of "Queer As Folk". Michael's a gay comic book store owner and diehard fan (and walking encyclopedia) of the whole superhero genre.
Trivia: Spoiler: This is one of only two Spider-Man movies to not end with a funeral. Spider-Man ended with Norman's funeral, Spider-Man 3 ended with Harry's, The Amazing Spider-Man ended with Captain Stacey's, and The Amazing Spider-Man 2 ends with Gwen Stacey's funeral. Marvel Studio's Spider-Man: Homecoming finally broke this trend again in 2017, making it the first Spider-Man film in ten years to not feature a funeral during the ending.