Continuity mistake: This error occurs in the scene where Scooby and Cyber Scooby are on the Log Ride, being chased by Jaguaro. The Scoobys have different colored collars on (green and red). Right before Jaguaro reaches them, it can be seen that both Scoobys are wearing red collars, when the "real" Scooby is supposed to wear a green collar. After Jaguaro falls from sight it cuts cut back to the two Scoobys and once again they have different colored collars.
Continuity mistake: When Daphne and Cyber Daphne are in the spooky house. They try to trap Creeper in the trap door, look carefully at the distance of the button,when Daffy presses it, it is right near the trap door. Before that, it was no where near the trap door.
Continuity mistake: in the final level, where Scooby and Cyber-Scooby are trying to defeat that cat-monster, Scooby has a green collar, while Cyberscoob has a red one. When they push the monster down the log plume, they give each other a high five. In that scene, both Scoobies are wearing red collars.
Continuity mistake: While the scientist is explaining how the blue virus-monster attacked him and his students, a flashback of the attack scene is shown. However, in this flashback, the telephone wires that grabbed him have changed in colour, thickness, and amount.