At the end the nasty man is out of bullets so the Bad girl throws him a bullet from her rifle. He puts the bullet in but fails to revolve the cylinder so of course he loses as you would have to thumb-cock and pull the trigger 3-4 times to get to the live ammo. Dumb, dead desperado.
At the end of the film before the credits the four girls are on a ridge riding off to the right of the screen. If you look closely at a horse on the bottom left hand side of the screen, you'll see his tail go up and you'll witness him performing an "act of nature."