Plot hole: The side mission "Fragile Alliance" has Batman helping Bane destroying containers full of Titan. After destroying the containers and returning to Bane, a small fight ensues followed by a cut scene of Batman trapping Bane. Bane has the drug Venom visibly going through his body the whole time so he could easily break out, but remains trapped through the rest of the game.
Revealing mistake: When Clayface disguised as Joker is holding Talia hostage in the movie theater, they are both standing on stage in front of the screen with a movie playing. However, they are not casting shadows on the screen. Also, no shadow appears on the screen when Clayface takes on his true form.
More mistakes in Batman: Arkham City
Trivia: Just before Protocol 10 starts, you end up in a giant fight with Joker and a good number of his goons. If you activate Detective Vision during this fight, you'll see that Joker doesn't have a skeleton. This is also true when he's holding Talia hostage in front of the move theater. This offers a hint to how the plot plays out.
Trivia: At the theater Clayface is watching "The Terror" a movie starring Jack Nicholson, who played the Joker in 1989's Batman.
Trivia: When doing the side-mission "Enigma Conundrum", after going to where the first Riddler room is located, look up and you will see a sign for a place called "The Black Canary." The Black Canary is another DC Superhero and member of The Justice League.
Mr. Freeze: You will bring me Nora or you will die.
Batman: It was all a lie. There's nothing wrong with you.
Joker: Nice of you to say. But you of all people should know there's plenty wrong with me.
Professor Hugo Strange: I feel I should thank you, capturing Bruce Wayne is so much easier than Batman.
Question: How did Hugo Strange find out that Bruce is really Batman?
Answer: Listen to the fourth Riddler interview tape. He did a psychological profile of Batman, then picked among candidates as to who could be Batman. He's also smart enough to realize only a rich man could afford all of Batman's gear and vehicles.
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Answer: Batman later in the game discovers that Hugo Strange has been supplying everybody with weapons, so he would have given Two-Face the vat of acid.