Continuity mistake: While in the train station in Iskenderun, Marcus passes by the same spot and the same people twice (watch the man with the hen on the left).
Continuity mistake: When Donovan turns into a living corpse the badge on his lapel disappears.
Continuity mistake: When Donovan drinks from the grail his left hand holds the base with extended fingers. A frame later they are bent.
Continuity mistake: In the catacombs, Indy finds the tomb and blows dust away from the shield, cleaning the sides of the engraved cross, but leaving the rest very dusty. A frame later, the whole cross is spotless.
Continuity mistake: While the propeller chops the boat, Indy grabs Kazim and lifts him up saying, "This is your last chance." But from the opposite angle Kazim is still seated.
Continuity mistake: When young Indy discovers the thieves in the caves, the pot on the right swaps from laying horizontal on the ground to slanted and back to horizontal, depending on the angle.
Continuity mistake: Young Indy grabs the Coronado cross, but from the next angle his hand is away about to grab it, and also its position has changed.
Continuity mistake: When young Indy says, "Cortés gave away the cross in 1920.", the thief is holding it with both hands. A frame later he holds it with just one hand.
Continuity mistake: When Indy escapes out of his office through the window, there are no shadows around. Half a second later, from a different angle, two long shadows have appeared underneath the window.
Continuity mistake: When Indiana's dad blows up the truck beside them during the chase, there's a big explosion. However, in the next shot showing Indiana and Ernst Vogel, the same explosion behind them is smaller.
Continuity mistake: At the very end of the movie, when they all ride off through a tunnel, you can see all sorts of tassels hanging off each of the horses. When they reach the end of the tunnel and are riding off into the sun, there are no tassels at all apparent.
