Continuity mistake: In the scene in the room with the pool table, Erik hits the cueball. One shot later, the cueball is back in its original position (not moving), and in the next shot, the cueball is gone and a red ball is rolling into the screen. (01:20:00 - 01:20:35)
Continuity mistake: In the scene where Stifler is kidnapped by the little people, the outside passenger mirror on the van changes between shots. Viewed in the shots outside the van, the mirror is in it's normal location. From shots inside the van, looking out, the mirror is pushed forward, making it impossible for the driver to see out of that mirror.
Continuity mistake: In the hospital scene the pudding cup in Stifler's hand keeps jumping from hand to hand whenever they go from close up to wide angle shot.
Continuity mistake: During the mud football scene, the referee calls full time and the score board shows briefly Stiffler's team score as zero. Then the next shot shows 28. (00:45:00)