Continuity mistake: When Holly tells Mario to put on his helmet, his medal disappears. (00:11:06)
Continuity mistake: When the Mario Bros. throw the net of Jelectros towards the Doom Sub, the yellow eyes on their Frog Suits turn white. (00:10:38)
Continuity mistake: When the Koopalings fire the nets, gloves appear on Toad. (00:08:26)
Continuity mistake: When King Koopa tells the Koopalings to fire the nets, Hop's hair turns completely pink, while his green eyelids turn black like his glasses. (00:08:21)
Continuity mistake: When Princess Toadstool tells Mario to come, the eyelashes on her Frog Suit disappear. (00:06:08)
Continuity mistake: Throughout the episode, King Mackerel's head changes from purple to skin colour, while his hands change from purple to green several times.
Continuity mistake: When Luigi says King Koopa will get even with him and his friends, his green eyes turn blue like Mario's. (00:05:21)
Continuity mistake: When Mario says he is afraid of the water, his blue eyes turn green like Luigi's. (00:04:57)
Continuity mistake: When Holly says Mario is safe, her blue and white bracelets turn completely white. (00:03:24)
Continuity mistake: When Mario's friends put him on a table, Princess Toadstool's Frog Suit covers her hands, despite not covering them in previous shots. (00:02:43)
Continuity mistake: When Mario saves the princess, gloves appear on her. This also happens when she and her friends leave to stop King Koopa, and when King Koopa starts firing nets at them. (00:01:52 - 00:07:33)
Continuity mistake: When Mario and his friends are dodging the wrenches, Toad's black eyebrows turn green like his Frog Suit. (00:01:21)
Continuity mistake: When King Koopa says Metroplis will be his, the green rings around the spikes on his shell turn orange like the spikes themselves. In the same shot, he speaks without moving his mouth. (00:01:00)
Continuity mistake: Throughout the episode, the nostrils on Princess Toadstool's Frog Suit disappear several times.