Other mistake: During "My Freeze Ray", there's a shot where Billy, Penny, and several other people open doors to the laundry machines in sequence. When Billy opens the door to his machine, you can see the reflection of everyone else just standing there, waiting for their cue to walk forward.
Continuity mistake: During "Brand New Day", at one point, Dr. Horrible stabs a picture of Captain Hammer attached to a dartboard with a handful of darts, all right in the center of the picture. A few shots later as Dr. Horrible sits, suddenly the darts are far-more spread out. (It's in the background and can be hard to see, but it's noticeable.)
Dr. Horrible: Oh goodness, look at my wrist, I have to go.
Trivia: The film was written during the 2007-2008 Writer's Guild strike, which served as an inspiration for the production. Director/Co-Writer Joss Whedon, whom took part in the strike, wanted to make an independent project that he could create without having to deal with the red-tape of studios, unions, etc. Essentially to avoid the same sort-of issues that caused the strike in the first place.
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