Factual error: In the scene when they are leaving the Pittsburgh Zoo, the sign indicates to turn right for Interstate 79. However Intestate 79 is about 15 miles to the west of the zoo. Also, it is suggested that Interstate 79 is the turnpike (a tolled road), when actually Interstate 76 is the turnpike and Interstate 79 is not tolled.
Plot hole: During the scene where John (Russell) decides he needs to break into the medical van, he watches an infamous YouTube video about using a tennis ball to open "car door locks." Unfortunately for John, the YouTube video was faked, and in reality was proven to be fake on the TV show MythBusters. (00:55:40 - 00:56:30)phiberoptik
Other mistake: Lara's hair is plain and pulled back in a pony tail when she first escapes. Within a short period of time, her hair becomes nicely styled but she would have needed a fair amount of time with a curling iron, hairspray, etc., for it to look so nice.